Surface biofunctionalization by covalent co-immobilization of oligopeptides

Immobilization of Subtilisin Carlsberg on Modified Silica Gel by Cross-linking and Covalent Binding Methods

Proteases are important enzymes that their role in various industries is undeniable. However, keeping enzymes stable during its activity in harsh conditions is so important. In this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and pHs was evaluated. First silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

Site-Directed Covalent Immobilization

Covalent Immobilization of Native Biomolecules

We have worked out a procedure for covalent binding of native biomacromolecules on flat gold surfaces for scanning probe microscopy in aqueous buffer solutions and for other nanotechnological applications, such as the direct measurement of interaction forces between immobilized macromolecules, of their elastomechanical properties, etc. It is based on the covalent immobilization of amino group-c...

Surface functionalization by covalent immobilization of an innovative carvacrol derivative to avoid fungal biofilm formation

Carvacrol, an aromatic terpenic compound, known to be antimicrobial was grafted onto gold surfaces via two strategies based on newly-synthesized cross-linkers involving either an ester bond which can be cleaved by microbial esterases, or a covalent ether link. Surface functionalizations were characterized at each step by reflection absorption infrared spectroscopy (RAIRS). The two functionalize...

Covalent Immobilization of Sapindus Peroxidase on Polyaniline

Covalent immobilization of peroxidase is convenient in continuous processes to improve them economically. In the present study, peroxidases from Sapindus mukorossi were immobilized and used for removal of 4-chlorophenol (4-CP) by the polymerization pathway. Five milligram of vacuum dried polyaniline-glutaraldehyde composite was incubated in 1ml sodium acetate buffer (50mM;pH 5.0) containing 0.5...